关于桐梓县提升烟叶收购站点规范管理的几点思考

为探索烟叶收购站点规范管理的可行路径和有效措施,通过走访调研,广泛听取烟草公司干部职工代表、烟农代表、合作社相关人员的意见,全面掌握了当前烟叶收购中存在的问题,提出烟叶收购站点规范管理“四个一”的对策建议。     

中国沙棘子叶叶肉细胞电流全细胞记录的研究

实验得出适合膜片钳实验的耐盐木本植物中国沙棘子叶原生质体的游离条件,即取当天脱壳的子叶,放入渗透浓度为0.88 mol/L,pH为5.8,酶液的组分为10 g/LCellulase R-10、0.5 g/LPectolyase Y-23、1.5 g/LMacrozymeR-10、1 g/L Hemicellulase、1 g/L BSA、1 g/L PVP-40、2 mmol/L CaCl2、10 mmol/L Mes和5 mmol/L Vc的溶液中,在26℃、黑暗条件下,静置4 h。并且利用Multiclamp 700B放大器和Clampex 9.2软件,记录到了该原生质体质膜全细胞内向K 通道电流(-459.137 pA及-290.527 pA)和非选择性阳离子通道的外向电流(8.036 pA)。这为沙棘属木本植物细胞的电生理特性及其耐盐性机制关系的研究奠定基础。     

基于政府采购方式下图书供应商的选择——以高校图书馆为例

分析了政府采购方式下选择图书供应商的优点及存在问题．并对政府采购方式下如何选择资信优良的图书供应商进行了探讨。     

馆商携手,合作双赢——一种新的图书采编模式

探讨了对图书供应商的考察和选择方法，各种采选方式的把握，图书加工和质量控制应注意的问题，对馆商合作的利弊进行了分析。     

高等学校图书馆文献资源招标和竞价采购的利弊分析

对高等学校图书馆文献资源实行招标和竞价采购的可行性、有利因素和不利结果进行阐述,提出加强高等学校文献采购的应对方法。     

Bufalin activates chloride channels in poorly differentiated nasopharyngeal carcinoma cells

AIM: To investigate the activation of chloride channels induced by bufalin and the properties of the channels in poorly differentiated nasopharyngeal carcinoma (CNE-2Z) cells. METHODS: The technique of whole-cell patch clamp was used to record the chloride currents and to analyze the characteristics of the currents in CNE-2Z cells.RESULTS: A chloride current was slowly activated by extracellular application of bufalin (1 μmol/L). The activation of the current was slower than that of the volume-activated chloride current, with an activation latency of(12.1±6.4) min. The reversal potential of the current was close to the calculated Cl^- equilibrium potential (E_Cl =-0.9 mV). The chloride current was outward-rectified and did not show significant time-dependent or voltage-dependent inactivation. The chloride channel blocker tamoxifen completely inhibited the outward and inward currents. The current was also completely inhibited by extra-cellular application of 47% hypertonic solution. CONCLUSION: Bufalin activates chloride channels and induces a chloride current in CNE-2Z cells. Compared with the volume-activated chloride current in CNE-2Z cells, the activation latency of the bufalin-induced current is longer and the outward rectification is more obvious.     

Modulation of K+ channels by Ca2+ and pH in regulatory volume decrease

It has been widely appreciated that animal cells rely on the mechanism of regulatory volume decrease(RVD) after swell in a hypotonic environment. Activation of K channels is crucial in the process of self-protection. There are a characterized increase in cytoplasmic Ca and a decrease in pH in the process of RVD in many cell types. Ca entry via transient receptor potential(TRP) channel is crucial for the cytoplasmic Ca increase, which in turn induces the decrease in pH.The increase in cytoplasmic Ca and decrease in pH activate or inhibit the activity of K channel, respectively. In this review, the regulatory network at cellular level between cytoplasmic Ca and pH, and the modulation of K channels by Ca and pH in the process of RVD are discussed.     

广州大学城绿化工程甲供苗采购招标工作经验浅析

本文通过对广州大学城园林绿化甲供苗招标采购工作流程的介绍及分析,总结出一些经验和针对实施过程中出现的问题提出一些解决的对策。     

供应链视角下的黑龙江乳业集团营销渠道冲突预警机制研究

基于供应链原理,以揭示供应链管理与营销渠道管理的关系为前提,在分析黑龙江乳业集团营销渠道冲突管理问题及症因的基础上,探索性地建立了服务于营销渠道冲突管理的供应链模型和基于供应链视角的营销渠道冲突管理预警机制,系统地说明了两个模型构建与运行的基本规律,提炼出营销渠道冲突预警机制既可以克服传统营销渠道冲突管理内在缺陷,又能够适应于新竞争模式带来外在压力的特有优势。     

半立方抛物线形明渠共轭水深的迭代算法

【目的】寻求半立方抛物线形明渠共轭水深的迭代计算方法。【方法】根据半立方抛物线形明渠断面的几何形态及棱柱体水平明渠水跃方程,推求得到半立方抛物线形明渠共轭水深的迭代计算公式,并从理论上证明其收敛性;通过对工程中不同流量Q与不同断面形状参数p多种组合情况下的共轭水深进行计算和趋势线拟合,建立计算共轭水深迭代初值的直接计算式。【结果】推导出半立方抛物线形渠道断面的水跃方程,并进而得到跃前水深、跃后水深的迭代计算公式,运用迭代初值直接计算式求出迭代初值,将该值代入共轭水深迭代计算公式,经过几步迭代便可收敛得到精度很高的共轭水深值。【结论】推求的半立方抛物线形明渠共轭水深迭代计算公式物理概念明确、计算简捷、精度高、适用范围广,可以满足工程实践要求。